CYANOCOBALAMIN ACID HYDRAZIDES AND THEIR CONJUGATES

摘要

The present invention provides cobalamin acid hydrazides of the general formula: B-CO-NH-NH-R-CO-NH-NH)xH (I) wherein B is a residue formed from a cobalamin by the splitting off of a -CONH2 group, R is a spacer and x is 0 or 1, as well as a process for the preparation thereof. The present invention also provides cobalamin conjugates of the general formula: B-d-CO-NH-NH-R-CO-NH)xN=GP (II) in which B, R and x have the above-given meanings and d signifies the position of -CO-NH-, as well as a process for the preparation thereof. These cobalamin conjugates are very suitable for use in immunoassays, especially for the determination of cyanocobalamin.