Hybridomas producing monoclonal antibodies to mono-, di- and trifucosylated type 2 chain

摘要

Hybridoma cell lines that produce monoclonal antibodies that differentially recognize glycolipids with mono-, di-, and trifucosylated type 2 chain structures are disclosed. The monoclonal antibodies can be used to detect specific types of tumor cells that are characterized by enrichment in mono-, di-, or trifucosylated type 2 chain structure. As such, the antibodies produced by the hybridoma cell lines are useful for diagnosis and treatment of human cancer. Also disclosed is an improved method of raising hybridoma cell lines by selecting the hybridomas by positive reactively with one or more fucosylated type 2 chain structures selected from the group consisting of III3FucnLc4, V3FucnLc6, III3FucnLc6, III3V3Fuc2nLc6, and III3V3VII3Fuc3nLc8.