摘要 |
PURPOSE:To obtain tannase, useful as a cleaning agent in beer production, in high yield by culturing a fungus belonging to the genus Aspergillus oryzae, capable of producing tannase in a medium containing tannic acid and a saccharide in a specific ratio, respectively. CONSTITUTION:A fungus [preferably variant of Aspergillus oryzae 19-30 (FERM P-9,3922]belonging to the genus Aspergillus, capable of producing tannase, is cultured in a medium containing 1-5% tannic acid and 3-7% saccharide and tannase is collected from the culture solution. The culture is preferably carried out under an aerobic condition at 25-35 deg.C culture temperature at initial pH 5.5-6 for 3-5 days. |