| 摘要 |
<p>Methods are provided for the identification and use of endogenous nucleic acid sequences for homologous recombination and targeted integration of heterologous nucleic acid sequences. These methods are useful for introduction of nucleic acid into cells or tissue for gene therapy, or for the preparation of transgenic animal. Methods are also provided for enhancing the yield of genes of interest from recombinant cell culture lines.</p> |
