| 摘要 |
The invention relates to an improved continuous countercurrent chromatography apparatus. The invention further relates to an improved method of countercurrent chromatography comprises filling a separation column with a first solvent, introducing into the column a sample solute to be separated, continuously pumping a second solvent into the column, the second solvent being substantially immiscible with the first solvent, maintaining the solvent flowing out of the column at a temperature effective to avoid clouding thereof, spectrophotometrically monitoring the temperature-maintained separating fractions flowing out of the column, and applying back-pressure to the solvent flowing into and out of the monitor.
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