METHOD FOR OBTAINING DNARECOMBINANT VECTOR, MICROORGANISM AND DNA, METHOD FOR OBTAINING RECOMBINANT PROTEIN AND RECOMBINANT PROTEIN

摘要

The invention concerns a DNA which contains (1) at least one of the two regions of the nucleotide sequence SEQ ID NO:1 coding for a protein with a Sau3AI methylase activity or for a protein with a Sau3AI endonuclease activity. (2) a sequence corresponding to the DNA from (1) within the scope of the degeneration of the genetic code or (3) a sequence which hybridizes under stringent hybridization conditions with a DNA from (1) or (2). In addition a vector is disclosed which contains a DNA according to the present invention, in particular under the control of a regulatable closed promoter, as well as microorganisms which are transformed with one or several vectors according to the present invention and the isolation of recombinant Sau3AI endonuclease from the transformed microorganisms.