发明名称 |
Colorimetric assay for detecting fungal cell envelope perturbations |
摘要 |
Methods of constructing and using gene expression systems to indicate the pertubation of fungal cell envelopes are provided. The recombinant genes produce indicator cleavage enzymes such as beta -galactosidase when galactose is able to enter the cell, despite the presence of glucose, through discontinuous cell envelopes. The indicator cleavage enzymes such as beta -galactosidase cleaves a colorimetric indicator placed in the media to produce an easily detected measurement of altered cell envelope permeability.
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申请公布号 |
US5168040(A) |
申请公布日期 |
1992.12.01 |
申请号 |
US19880168831 |
申请日期 |
1988.03.16 |
申请人 |
SCHERING CORPORATION |
发明人 |
RYAN, MICHAEL J.;LOTVIN, JASON A.;SMITH, ELIZABETH B.;SHAW, KAREN J. |
分类号 |
C12Q1/02;C12Q1/04 |
主分类号 |
C12Q1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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