BIOSENSOR AND SEPARATION AND QUANTIFICATION METHOD USING THE SAME

摘要

PURPOSE:To simply and economically exclude the electrochemical or chemical obstructing action due to the reductive substance present in a sample by arranging a substance containing a physiologically active substance to the region separated from an electrode. CONSTITUTION:A spacer 3 having a sample receiving space 3' and a lid 4 are bonded to the electrode 2 formed on a substrate 1 and a substance containing a physiologically active substance is arranged to the region 6 separated from the electrode 2. The sample is introduced into the space 3' from an opening 7 to be measured. First measurement is performed at the beginning of the supply of the sample and second measurement is performed after the elapse of a sufficient time. The first measured value is a value before the product from the objective substance reacted with the physiologically active substance reaches the electrode and the density of the obstructing substance coexisting in the sample is quantified from said value. The second measured value is the value due to both of them and the concn. of the sum of the objective substance and the obstructing substance is quantified from said value. Therefore, by subtracting the former from the latter, the concn. of the objective substance is calculated.