METHODS FOR PRODUCING MEMBERS OF SPECIFIC BINDING PAIRS

摘要

The invention provides methods and kits for producing specific binding pairs (sbp) members. Populations of polypeptide chain components of sbp members are combined to form libraries of sbps displayed by secreted replicable genetic display pack- ages (rgdp). At least one of the polypeptide chains is expressed as a fusion with a component of an rgdp which thereby displays that polypeptide chain at the surface of rgdp. At least one population of polypeptide chains is expressed from nucleic acid which is capable of being packaged using a component of an rgdp, whereby the genet ic material of rgdps produced encodes a polypep- tide chain. The methods enable production of libraries of multimeric sbp members from a very large number of possible combina- tions. In one embodiment of the invention a method emplays "chain shuffling" in the production of sbp members of desired spe- cificity for a counterpart sbp member. Selection procedures are also described.