RECOMBINANT DNA CODING FOR SIGNAL PEPTIDE, SELECTIVE INTERACTING POLYPEPTIDE AND MEMBRANE ANCHORING SEQUENCE

摘要

Recombinant DNA sequence comprising a first DNA fragment coding for a first amino acid sequence operating as a signal peptide operable in a Gram positive host, operatively linked to a second DNA fragment coding for a second amino acid sequence not naturally found on the surface of Gram positive bacteria and capable of selective interaction, said second DNA fragment being operatively linked to a third DNA fragment coding for a third amino acid sequence operable in a Gram positive host as a cell wall spanning and membrane anchoring sequence; an expression vector or plasmid containing such recombinant DNA sequence; Gram positive bacterial cell containing such vector or plasmid; and a process for selective isolation or identification of Gram positive bacterial cells.