METHOD OF SEPARATING PROTEIN AND APPARATUS THEREFOR.

摘要

A method of precisely separating a specified protein from a solution of proteins in a short time, which comprises packing a gel, prepared by immobilizing a DNA having a sequence which specifically combines with a restriction enzyme Eco RI through bonding to a silica particle at its terminal, in a small-diameter chromatographic column (8), pouring a protein solution containing Eco RI into the column to combine the gel with Eco RI, pouring a mixture of two buffers into the column while giving thereto a concentration gradient with the lapse of time, eluting Eco RI when the concentration of the buffer reaches a specified value, developing the buffer eluted from the column on a nitrocellulose membrane, and taking out a portion containing Eco RI.