PURIFICATION OF IL-2 BY RECEPTER-AFFINITY CHROMATOGRAPHY

摘要

IL-2 receptor affinity adsorbents which contain soluble IL-2 receptor molecules which are immobilised on a solid carrier are prepared as described and are used in methods for the purification of interleukin-2 (IL-2) from various sources, e.g. from synthetic mixtures, from culture media which are conditioned by natural IL-2-producing cells, and from mammalian or bacterial cells which are transformed with a vector which is able to express IL-2. Purified IL-2 can be isolated in one step by the use of these affinity adsorbents. The IL-2 purified in this way is very largely free of aggregated forms which are often present when other purification methods are used.