| 摘要 |
PURPOSE:To efficiently produce the subject optically active (R)-2-hydroxy-4- phenylbutyric acid from 2-oxo-4-phenylbutyric acid. CONSTITUTION:An enzyme obtained by extraction and purification from a microorganism belonging to Leuconostic genus is allowed to act on 2-oxo-4- phenylbutyric acid on the presence of reduced type nicotinamde adenine dinucleotide phosphate NADPH as a coenzyme to asymmetrically reduce it. The above-mentioned enzyme has following physicochemical properties; Optimum pH: about 6.0, Stable pH region: 6-7, Optimum temperature: about 45 deg.C. Thermal stability: <=40 deg.C, Michaelis constant value Km for 2-oxo-4-phenylbutyric acid: 0.29mM, Molecular weight: 46000 and Inhibitor: mercury chloride and mercury para-chlorobenzoate. |
