| 摘要 |
PURPOSE:To obtain WF11243 substance which is a new substance having excellent antimycotic action and useful for the prevention and treatment of various diseases caused by true fungi by culturing a WF11243 substance-producing microorganisms belonging to true fungi and collecting the product from the culture liquid. CONSTITUTION:A WF11243 substance-producing strain No.11243 (FERM BP-3373) belonging to true fungi is inoculated in a nutrient source containing glucose, sucrose, etc., as assimilable carbon source and oat meal, yeast extract, etc., as nitrogen source and cultured in a jar fermenter under aerobic condition at 25 deg.C for 4 days. The cultured product is added with acetone, stirred and filtered to obtain an extract. The extract liquid is added with water and purified by successively fractionating with an adsorbent resin column and a reverse-phase carrier column to obtain the objective WF11243 substance which is white powder having a melting point of 182-187 deg.C and a specific rotation [alpha]<25>D of +29 deg. (c=1.5, methanol). |
