| 摘要 |
PURPOSE:To obtain a mouse-human chimera antibody useful as a cancer marker, a diagnostic and treating agent for cancer, etc., by binding a specific site of a variable range of mouse antibody to be specifically linked to a human carcinoembryonic antigen to a normal range of human antibody. CONSTITUTION:Messenger RNA is extracted by a conventional procedure from a hybridoma which is prepared by immunizing BALB/c mouse with human carcinoembryonic antigen (human CEA) to give an antibody-forming cell and fusing the antibody-forming cell with a mouse myeloma cell. Cyclic DNA is synthesized from the mRNA, a cDNA library is prepared by a conventional procedure, a DNA encoding an antibody heavy chain variable range and a light chain variable rang of mouse is selected by a probe, the DNA is accurately linked to a DNA encoding a stationary range of human antibody so as not to cause frame shift in amino acid substitution to give the objective mouse- human chimera antibody having an amino acid sequence wherein the heavy chain range of mouse antibody specific to human CEA is shown by the 1st to the 120th of formula I and the light chain range is shown by the 1st to the 109th of formula II. |
