Method for lysing hard liposomes using polyethyleneglycol mono-n-alkyl ethers

摘要

A method for rapidly lysing liposomes having transition temperatures in the range of 35 DEG C. to 65 DEG C. is provided. Such liposomes are treated with a surfactant including polyethyleneglycol mono-n-alkyl ethers such as C12H25O(CH2CH2O)nH where n=9-10, a nine mole ethylene oxide adduct of a blend of n-dodecanol, n-tetradecanol and n-hexadecanol, or other appropriate polyethyleneglycol mono-n-alkyl ether capable of causing rapid lysis. The method is applicable to fluorescence immunoassay procedures.