| 摘要 |
The present invention relates to an electrophoretic method to effect differential net migration of electrically charged macromolecules through a gel support in a single dimension, the extent of said migration being dependent on molecular size, which method comprises subjecting electrically charged macromolecules applied to a gel support to an electric field oriented along a single axis within the gel for a time period sufficient to effect migration in the direction of the oriented field and form a separation pattern in order of the respective molecular weights of the macromolecules in a distance of about 0.5 to about 20 millimeters, said gel support having a restrictive pore diameter relative to the effective radius of the macromolecule, said electric field being applied in an amount of about 5 to about 500 volts per millimeter of gel support along the axis length, and said gel support having a width perpendicular to said axis of about 0.05 to 2.0 millimeters. |
