DNA CONSTRUCT FOR IN VIVO EXPRESSION OF A HUMAN GENE

摘要

A plasmid consists essentially of a small pCMV4 expression vector including a coding sequence of human alpha-1 antitrypsin incorporated therein. The present invention further provides a method for delivering the gene to a patient, the mechanism including a liposome including the expression plasmid incorporated therein, the plasmid being capable of expression of the gene extrachromosomally in the cells of a target tissue and being unincorporable into the chromosome of the cells of the target tissue. The present invention further provides a primer for inserting the coding sequence of the protein into the expression vector. Finally, the present invention provides a method of treatment for deficiency of a gene product in cells of a target tissue by using the novel liposome.