Use of baculovirus early promoters for expression of foreign genes in stably transformed insect cells or recombinant baculoviruses

摘要

This invention details a novel and unique plasmid vector for the dual purposes of either producing transformed insect cell clones or recombinant baculoviruses. The transformed insect cell clones will continuously and permanently produce an efficiently processed desired foreign gene product. The recombinant baculoviruses will transiently express the desired foreign gene during immediate early phase of infection. This unique vector employs promoters from two different immediate early baculovirus genes along with the natural occurring polyhedrin promoter and gene. This unique combination creates a situation where the virus is highly infectious in vivo and resistant to inactivation in nature, because it is occlusion positive, and expresses the foreign gene product during immediate early phase of infection. Therefore this virus would be very effective as a delivery system of pesticides.