发明名称 |
Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms |
摘要 |
A process for recovering highly pure, recombinant IL-2 from transformed microorganisms in which the cells are disrupted; impure recombinant IL-2 is isolated in the form of refractile bodies from the disruptate; the impure IL-2 is dissolved and denatured with at least 6M guanidine hydrochloride containing a reducing agent; the reduced IL-2 is precipitated and resolubilized; the reduced solubilized IL-2 therein is oxidized by a controlled oxidation; the oxidized IL-2 is refolded by reducing the concentration of guanidine hydrochloride in the solution; and the oxidized, refolded IL-2 is further purified by ion exchange chromatography or hydrophobic interaction chromatography and ion exchange chromatography.
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申请公布号 |
US5162507(A) |
申请公布日期 |
1992.11.10 |
申请号 |
US19890406365 |
申请日期 |
1989.09.12 |
申请人 |
CETUS CORPORATION |
发明人 |
WOLFE, SIDNEY N.;DORIN, GLENN J.;DAVIS, JOHN T.;SMITH, FLINT;LIM, AMY;WEISSBURG, ROBERT |
分类号 |
C07K1/113;C07K14/55 |
主分类号 |
C07K1/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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