ENZYMATIC RESOLUTION OF .ALPHA.-TERTIARY CARBOXYLIC ACID ESTERS

摘要

ENZYMATIC RESOLUTION OF .alpha.-TERTIARY CARBOXYLIC ACID ESTERS A method for the resolution of .alpha.-tertiary carboxylic acid esters by partial hydrolysis is disclosed. The partial hydrolysis is carried out by contacting the esters with an aqueous solution in the presence of an enzyme, and the hydrolysis product is separated from unreacted starting material to obtain the hydrolysis product or the unreacted starting material in enantiomerically enriched form. A novel, purified enzyme which is useful in this method, and a nucleotide sequence encoding this enzyme, are also described.