DETECTION OF 3'-AZIDO-3'-DEOXYTHYMIDINE RESISTANCE

摘要

An assay for detecting the genotype of mutant and wild-type alleles at multiple loci associated with resistance to the drug AZT utilizes a series of probes which anneal to an amplified region of the HIV-1 pol gene. The assay format which involves amplification of a rare nucleic acid followed by capture on a solid phase, and detection of the captured sequences by a labelled probe, is applicable to assays for resistance to other drugs involving point mutations.