| 摘要 |
The present invention relates to a method for enhancing the amount of recombinant protein recovered after fermentation of bacteria containing vectors encoding the recombinant protein. The method is not limited by a required induction of the cloned gene by addition of exogenous inducers. Using the methods of the invention, bacterial recombinant protein production can exceed a level of 20 % of the total soluble protein expressed. The methods of the invention are demonstrated on bacterial cytochrome P450 and on bacterial electron transfer proteins typically difficult to express to commercially feasible levels. |
