| 摘要 |
PURPOSE:To obtain the subject new peptide conjugate capable of inhibiting infiltration of a cancer cell during a process of cancer cell metastasis and useful as a cancer metastasis inhibitor by chemically bonding a peptide having a specified amino acid sequence to chondroitin sulfate or hyaluronic acid. CONSTITUTION:A peptide is synthesized using an automatic peptide synthesizer by the Merribield solid phase synthesis method and the resultant product is subsequently subjected to high-performance chromatography to elute the peptide by the linear concentration gradient method. The peptide having an amino acid sequence represented by the formula (Glx is Glu or Gln) can be obtained thereby. The above-obtained peptide and chondroitin sulfate or hyaluronic acid respectively derived from cow trachea are then dissolved in distilled water and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, etc., is added thereto. Condensation reaction is then carried out and the resultant reaction product is subsequently purified by high-performance chromatography, thus giving the objective peptide conjugate useful as a cancer metastasis inhibitor, etc. |
