METHOD FOR DETECTION OF DWARF VIRUS

摘要

A DNA probe for early diagnosing black line dwarf disease virus (I) is prepd. by (a) separating the (I) from a green leafhopper larva infected with the (I), (b) preparing cDNA library of the (I), (c) ligating the obtd. cDNA with rambda gtll vector to obtain the recombinant rambda gtll phage DNA (II), (d) inserting the obtd. (II) into pUC19 and transforming into E. coli JM101, and (e) collecting the recombinant clone. The obtd. DNA probe is useful for the early diagnosis of a green leafhopper larba infected with the (I).