| 摘要 |
<p>A method for detecting chitin-containing organisms such as fungi, yeast and insects in a sample attached to a solid phase, by detecting the presence of chitin by using enzymes which specifically bind chitin is disclosed. The method is preferably carried out by passing a sample which contains chitin through a membrane in order to capture and adhere the chitinous material on the membrane. A chitin-binding enzyme such as chitinase or lysozyme is added under conditions which allow it to bind to the chitin on the membrane. A signal generating compound is either directly conjugated to the chitinase (or lysozyme) or indirectly conjugated to either an anti-chitinase (or lysozyme) antibody or to an anti IgG antibody (which binds to the anti-chitinase antibody) to detect the chitin-chitinase complex. Reagents for the signal generating compound then are added to produce a detection signal which indicates the presence of the chitin. Kits which contain the necessary materials for carrying out the methods of the invention are also disclosed as are novel conjugates.</p> |
