| 摘要 |
PURPOSE:To obtain a new cardenolide compound, composed of a glycoside, extracted and isolated from a herb medicine teen-ped grown in Thailand, having cardiotonic activity and useful as a therapeutic agent for improving cardiac insufficiency, arrhythmia, etc. CONSTITUTION:Extracting operation of teen-ped grown in Thailand is performed using methanol and the resultant extract solution is then concentrated under reduced pressure to provide a concentrate, which is subsequently distributed with n-hexane. The obtained methanol layer is separated and the methanol is distilled away under reduced pressure to distribute the residue with ethyl acetate and water. The aqueous layer is further distributed with n-butanol to afford an n-butanol fraction, which is subsequently fractionated by centrifugal liquid-liquid partition chromatography using 5:5:1:4 composition of chloroform : methanol : n-butanol : water of a distributing solution. Thereby, the objective 3-[(O-glucopyranosyl-(1 6)-O-glucopyranosyl-(1 4)-6-deoxy-3-O-methyl- glucopyranosyl)oxy]-7-ene-14-hydroxycard-20(22)-enolide expressed by the formula. |
