摘要 |
The sensitivity of refractive gradient detectors for detecting refractive index gradients in samples flowing through samples chambers is increased by detecting the gradients formed in the direction perpendicular to the direction of flow of the sample through the sample chamber and by detecting such gradients simultaneously intermediate the central axis and sides of the sample chamber on opposite sides of the central axis. A preferred detector uses two spaced, parallel probe light beams passing through the sample chamber between the central axis and sides of the chamber on opposite sides of the axis, with each beam preferably centered about half way between the axis and the respective side. A sensor detects relative movement of the probe beams caused by a refractive index gradient in the sample but preferably compensates for parallel movement of the parallel light beams caused by positional instability or mechanical movement of the light source. The sensor produces an output signal proportional to the refractive index gradient detected.
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