Prodn. and use of collagen - in which animal intestines with base material are mixed with ice water at pH 5.5, heated and hydrolysed at pH 10.5 using proteolytic enzyme

摘要

Native collagen is produced from animal intestines, stomach, rumen, lungs and udders, and the base material is cleaned and immersed inice water, with the pH regulated to approximately 5.5. The mixt. of base material and ice water is ground, and then fuether water is added so that the ground mixt. contains approximately equal wt. parts of base material and water. The mixt. is then heated to 40-42 deg.C and the pH is regulated to pref. 10.5, after which a proteolytic enzyme is added in an amt. corresp. to 50 Anson units per kg so that hydrolysis of proteins other than collagen takes place, maintaining the pH value by addition of alkali until the hydrolysis is ready and the alkali is no longer consumed. The pH is then regulated to 5.5. by the addn. of acid, and then the sepd. collagen is removed and used. The pH is regulated at al stages with the aid or hydrochloric acid, citric acid or lactic acid.