| 摘要 |
PURPOSE:To provide a new peptide containing an amino acid sequence such as L-leucine, L-glutamine, L-proline, etc., having hypotensive action and useful as a treating agent for essential hypertension, etc., as an angiotensinase inhibitor. CONSTITUTION:Wheat gluten is added with distilled water, heat-treated at 121 deg.C for 10min, added with 1M sodium citrate buffer solution (pH 2.0), adjusted to pH 2.0 with 1N-HC1, added with molsin (an acidic protease) and stirred at 30 deg.C for 44hr to effect enzymatic reaction. The obtained hydrolyzate is heated at 100 deg.C for 10min to deactivate the residual enzyme and the insoluble materials are removed by centrifugal separation to obtain a filtrate. The filtrate is condensed and then purified by successively subjecting to gel-filtration chromatography and reversed phase high-performance liquid chromatography to obtain the objective peptide expressed by formula (R<1> is H or amino acid residue; R<2> is hydroxyl or amino acid residue) and having an amino acid polymerization degree of 3-6. |
