| 摘要 |
The optical detection of the concentration of a specific substance in a liquid or a gas by the utilization of the quenching of a phosphor by the specific substance or a substance derived from the specific substance is attained with high accuracy by a method and apparatus which comprises causing the system of determination to induce the presence of a first phosphor (A) quenchable by the specific substance or a substance derived from the specific substance and a second phosphor (B) substantially unquenchable by the specific substance or a substance derived from the specific substance, measuring the light signal corresponding to the intensity (IfA) of emission from the first phosphor (A) and the light signal corresponding to the intensity (IfB) of emission from the second phosphor (B), and calculating the ratio between the light signals thereby offsetting variable factors relative to the intensities of emission. The first phosphor is selected from the group consisting of tris(2,2'-bipyridine) ruthenium (II) complex, tris(1,10'-phenanthroline) ruthenium (II) complex, 1-pyrenebutyric acid and salts thereof, pyrene, aminopyrine, perylene, perylene dibutylate, and 2, 7-dichlorofluorescein. The second phosphor is selected from the group consisting of proflavine sulfate, fluorescein, and eosin.
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