METHOD AND DEVICE FOR THE SEPARATION AND ISOLATION OF BLOOD OR BONE MARROW COMPONENTS

摘要

Method and apparatus for the separation and isolation of blood components are described. After separation and during centrifuging a separated blood component is transferred from a source reservoir into a tube oriented against a centrifugal gradient. A cap converts the separated layer from the reservoir into a columnar body and the centrifugal gradient prevents mixing of the columnar body in the tube during further centrifuging. In one embodiment the tube has a volume sufficient to hold a desired fractional component from the source reservoir, and a diameter small enough to prevent mixing of the component when the gradient is removed. A support holds the conduit wound about a spool transverse to the iso-g-lines of the centrifuge. The support may be removably affixed to the cap. In a preferred embodiment the source reservoir has a flexible wall, and pressure of the wall against an elevation maintains pressure in the fluid system. Flow blocking and flow regulating means are shown for delivering small fractional components to the tube or to a receiving reservoir at a desired rate. In another embodiment a make-up fluid reservoir maintains fluid pressure and a peristaltic pump controls the transfer of separated components. A table with movable clamps is also shown for isolating the fractions in the tube after centrifuging.