| 摘要 |
<p>PURPOSE:To obtain a new phosphopeptide, usable as an antigen for preparing an antibody, specifically reactive with fibrous protein paired helical filament (PHF) accumulating in the brain of patients suffering from Alzheimer geriatric dementia and capable of detecting the PHF and useful for producing a diagnostic agent for the Alzheimer type geriatric dementia. CONSTITUTION:The objective new phosphopeptide expressed by the formula [X is PO(OH)2 group] is obtained by adding bis[(2-methylphenyl)oxy]phosphoryl chloride prepared from phosphoryl chloride and 2-methylphenol to a solution of a compound expressed by the formula Boc-Ser-OBzl (Boc is tert. butyloxycarbonyl; Bzl is benzyl) in pyridine, converting hydroxyl groups into phosphoric acid esters, removing the carboxyl-protecting groups thereof, providing an (alpha-amino-protected amino acid for solid-phase synthesis, then synthesizing a protected peptide chain according to an amino acid sequence on a support, subsequently eliminating the peptide from the support and removing all the protecting groups.</p> |
