| 摘要 |
PURPOSE:To prevent the deactivation of enzyme with heavy metal (e.g. Ag) in an analyzer utilizing an SH enzyme, by contacting the immobilized enzyme constantly with a low molecular SH compound during the pause of the measurement. CONSTITUTION:The cell 19 is furnished with an enzyme electrode (having an enzyme membrane immobilized with an enzyme such as urease, as a sensitive membrane), a reference electrode 2 and an earth electrode 3 in the flow path in the order. The specimen supplied from the injector 18 is carried by the carrier buffer liquid 15 and transferred to the cell 19 through the thin tube 14 and the feed pump 17. A signal corresponding to the concentration of the material (e.g. urea) to be determined is generated by the electrode, and is transferred via the preamplifier 20 and recorded with the recorder 21. The carrier buffer solution in the above process is the one containing about 0.1-100mM of a compound containing SH group (e.g. mercaptoethanol, glutathion, etc.). |
