A method of isolating and purifying galactose oxidase from a fungus media is disclosed. The supernatant liquid from the fermentation broth is subjected to ultrafiltration and then separated on a carboxy-sulfon cation exchange column using high performance liquid chromatography. Copper ions must be present to insure enzyme activity.
申请公布号
US5149646(A)
申请公布日期
1992.09.22
申请号
US19910807010
申请日期
1991.12.13
申请人
THE PROCTER & GAMBLE COMPANY
发明人
CRANE, LAURA J.;MAZUR, ADAM W.;NAU, DAVID R.;KLUESENER, BERNARD W.