Monophenotypic xenograft of megakaryocytic lineage and origin

摘要

A continuous cell line of megakaryocytic origin is disclosed. This novel continuous cell line has been established in athymic nude mice. The cells of the established cell line monophenotypic for megakaryocytes is characterized as having multilobed nuclei and granular cytoplasm and being reactive for Factor VIII antigen, glycoprotein IIB-IIIa complex antigen and platelet peroxidase. The established cell line of this invention generates in isolatable quantities platelet-like factor IV, FGF-like, beta -thromboglobulin-like and TGF- beta -like growth factors. Methods for cultivating the established cell line, in a nude animal and isolating and purifying the proteins are also disclosed.