A biosensor utilizing enzyme and a method for producing the same.

摘要

The biosensor of the present application determines an analyte (eq fructose) contained in a sample liquid by reducing electron acceptors using electrons generated in a reaction of the analyte with an enzyme and then by measuring the reduced amount of the electron acceptors electrochemically. The biosensor has an electrical insulating substrate (1), an electrode system (14) including at least a working electrode (4) and a counter electrode (5), a reaction layer (15) including the enzyme (7) provided on the electrode system and a hydrogen ion concentration (pH) control layer (11), and the reaction layer is in contact with the electrode system. According to the present application, the pH of the sample can be optimised for the type of the enzyme contained in the reaction layer, without prior addition of pH buffer to the sample liquid. Thus, the specific analyte contained in the sample liquid can be easily determined with accuracy and speed. <IMAGE>