Water-soluble tetraazaporphins and fluorochrome for labeling.

摘要

A fluorochrome for labelling comprises a compound of formula (II): <CHEM> wherein M is H2, Mg, Al, Si, P, Zn, Ga, Ge or Sc; Y is a halogen atom, -OR<1>, -NR<2>2 (in which the groups R<2> may be the same or different) or -SR<3> wherein R<1>, R<2> and R<3> are independently selected from hydrogen, alkyl groups, alkyl groups having one or more hydrophilic substituents, aryl groups, aryl groups having one or more hydrophilic substituents, aralkyl groups, aralkyl groups having one or more hydrophilic substituents, acyl groups, acyl groups having one or more hydrophilic substituents, silyl groups, silyl groups having one or more hydrophilic substituents, phosphorus-atom-containing groups and phosphorus-atom-containing groups having one or more hydrophilic substituents; p is zero or an integer of 1 or 2 indicating the number of Y's bonded to M and when p is 2 the groups Y are the same or different; the groups A are the same or different and each is a fused di- or poly-cyclic aromatic group having m substituents X<1>Q (X<1> is a bond, oxygen, sulfur, nitrogen, phosphorus, silicon, selenium, NH.CO, NH.PO2, NH.SO2, O.CO, O.SO2, O.PO2, S.CO, S.SO2, S.PO2, CO, SO2 or PO2; Q is a saturated or unsaturated hydrocarbon group or a heterocyclic group) and the numbers m are the same or different and each is an integer of 1 to 4; the numbers n are the same or different and each is zero or an integer of 1 or more, provided that at least one n is an integer of 1 or more; the substituents EZ are the same or different and each is bonded to an aromatic ring of group A directly or via a group Q on an aromatic group A (wherein (i) E is a cationic group and Z is an anion, (ii) E is an anionic group and Z is a cation or (iii) E is a bonded group containing a polyethylene glycol residue, a polyether residue, a polyamine residue, a polyalcohol residue or a polycarboxylic acid residue and Z is absent). Most of these fluorochromes are novel. Labelled substances and reagents comprising these fluorochromes and analytical uses of these florochromes are also provided.