NOVEL SHUTTLE VECTOR

摘要

pBlurescript II KS+ multiple cloning site-containing shuttle vector is claimed. pBluescript II KS+ multiple cloning site is replicated in corynebacterium glutamicum and/or E. coli pACYC 177 and PCB1 are each cut by BamHI and BCII and then ligated by T4 DNA lugase. And the multiple cloning site is introduced into pECCG1. The new shuttle vector is useful for making genome library and subcloning.