| 摘要 |
<p>Complementary DNA (cDNA) fragments encoding the Fab and Fv portion of the light and heavy chains of a murine monoclonal antibody (mAb) were prepared from hybridoma messenger RNA (mRNA). The mAb is specific for the intercellular adhesion molecule-1 (ICAM-1). Furthermore, the mAb blocks the attachment of human rhinovirus (HRV) particles to their cellular receptor, ICAM-1. Fab and Fv cDNA were molecularly cloned and expressed in a recombinant host, purified, and shown to be biologically active in that they bind to ICAM-1, and block attachment of ligands to ICAM-1, including HRV. <IMAGE></p> |
