摘要 |
Methods and apparatus for the rapid detection of mycobacteria in biological fluids are disclosed. The methods involve applying a biological fluid, one or more monoclonal antibodies selectively specific for an antigenic component of mycobacteria, and a labeled immunological reagent to a solid phase. The mycobacteria contained within the biological fluid are bound to the solid phase without the need for chemical fixation techniques. The solid phase is preferably nitrocellulose with a pore size greater than 0.22 microns and less than about 1.0 microns. Test kits are also described. |