摘要 |
PURPOSE:To resolve a (+ or -)-chrysanthemum-monocarboxylic acid ester into optically active chrysanthemum-monocarboxylic acid and its antipode ester, by subjecting a (+ or -)-chrysanthemum-monocarboxylic acid ester to assymetric hydrolysis with esterase produced by a bacterium. CONSTITUTION:A (+ or -)-chrysanthemum-monocarboxylic acid ester shown by the formula (R is lower alkyl group) is asymmetrically hydrolyzed with esterase obtained by a bacterium such as Arthrobacter globiformis IFO12958 belonging to the genus Arthrobacter, Flavobacterium, Rhodotorula, Rhodosporidium, Nocardia, or Thermomyces, capable of subjecting the (+ or -)-chrysanthemum shown by the formula to asymmetric hydrolysis at 20-50 deg.C at about 7pH, liberated optically active chrysanthemum-monocarboxylic acid and an unreacted ester are separated and recovered. |