METHOD FOR ASSAYING 1,5-ANHYDROGLUCITOL AND KIT THEREFOR

摘要

This invention relates to a novel method for assaying 1,5-anhydroglucitol, which is expected to serve as a marker for diabetes, and a kit therefor. More particularly, it relates to: (1) a method for assaying 1,5-anhydroglucitol, which comprises selectively removing sugars from a specimen containing 1,5anhydroglucitol; allowing pyranose oxidase or L-sorbose oxidase to act on 1,5anhydroglucitol contained in the sample thus obtained in the presence of oxygen; and determining 1,5-anhydroglucitol from the amount of the hydrogen peroxide thus formed; and (2) a reagent kit for assaying 1,5-anhydro-glucitol which comprises an agent for removing sugars, a reagent for detecting hydrogen peroxide and an enzyme for oxidizing 1,5-anhydroglucitol.