ENHANCED EXPRESSION AND STABILIZATION OF GENE PRODUCTS IN MICROORGANISMS USING SHORT SELECTED LEADER SIGNAL SEQUENCES

摘要

TITLE ENHANCED EXPRESSION AND STABILIZATION OF GENE PRODUCTS IN MICROORGANISMS USING SHORT SELECTED LEADER SIGNAL SEQUENCES INVENTORS Saran A. Narang Winq L. Sung This invention concerns the employment of DNA sequences comprising (a) a sequence coding for a desired polypeptide, and; (b) a selected leader signal sequence coding for a range of from a tri-peptide to about one third of the size of peptide coded by sequence (a), said selected leader signal sequence being chosen to enhance the effective expression of, and/or stabilize the polypeptide coded by sequences (a) and (b) when said DNA sequence resides and is expressed in a foreign host microorganism. The polypeptide products of sequences (a) and (b) preferably should be readily cleavable from each other to allow for simple purification of such products.