GENE CODING ALCOHOL DEHYDROGENASE

摘要

PURPOSE:To make it possible to efficiently obtain an alcohol by culturing transformant transformed with a manifestation vector containing a specific gene. CONSTITUTION:A bacterial cell obtained by culturing Bacillus stearothermophilusu NCA 1503 strain is extracted to afford chromosome DAN (A). Then the ingredient A is subjected to restriction enzyme treatment to produce a plasmid vector pTBAD35 (B) exhibiting restriction enzyme-cut map having 3.5Kb BamHI fragment and containing a gene coding alcohol dehydrogenase (ANH). Then the ingredient B is introduced into the above- mentioned NCA 1503 strain or B. subtilis strain to afford a transformant (C). Then the transformant C is cultured to produce ADH. On the one hand, a transformant obtained by introducing DNA for changing an amino acid constituting ADH is cultured to produce a modified ADH capable of shifting optimum pH to a basic side. As necessary the transformant C is cultured to produced an alcohol.