| 摘要 |
<p>A method for measuring luminescence in an assay by luminescence is described wherein certain disturbances due to the measuring medium can be corrected. The method is characterized in that it employs at least one luminescent tracer compound and a luminescent compound used as an internal reference which, when exposed to the same excitation wavelength, are capable of emission at different wavelengths, μ2 et μ1 respectively, either by direct luminescence or by induction of luminescent emission, and that the measurement of tracer compound luminescence at wavelength μ2 is corrected on the basis of the measurement of reference compound luminescence at wavelength μ1. The use of the method in a homogeneous analyte detection and/or determination process, and a device for its implementation, are also described.</p> |
