METHODS FOR ASSAYING PROTEINASES AND PROTEINASE INHIBITORS

摘要

Methods are provided for determining proteinases and proteinase inhibitors based on the ability of phenanthradine coumpounds to intercalate between the bases of DNA with a resulting increase in their fluorescence. The DNA is complexed with a protein to block binding of the phenanthradine. Proteinase activity degrades the complexed protein, exposing DNA to phenanthradine binding accompanied by increased fluorescence.