A METHOD OF PRODUCING AUTOANTIBODIES

摘要

A method of producing autoantibodies which are reactive with an antigen characteristic of an autoimmune disease, the method comprising a) administering lymphocytes from a donor individual suffering from an autoimmune disease to immunodeficient mammals, b) after a period of time sufficient to permit reconstitution of immune cells in at least one of said mammals, selecting a reconstituted mammal producing the desired autoantibodies, c) immortalizing B-lymphocytes of the selected mammal before or after isolating lymphocytes therefrom, and d) culturing the immortalized B-lymphocytes under conditions permitting their production of the desired autoantibodies and recovering the resulting autoantibodies and recovering the resulting autoantibodies from the culture.