发明名称 |
Determn. of lactose repressor over-producing microorganisms - using a phage with a marker gene contg. C-terminal with a lactose operator and promoter which forms an anti-sense RNA of the marker |
摘要 |
The microorganism are transfected with a bacteriophage carrying a detectable marker joined at its C-terminus with a lactose-promoter/operator which is transcribed as an anti-sense RNA of the marker. The expression prods. are detected. Pref. the phage is Lambda or its derivs., and the marker is the xylE-gene of Pseudomonas (P.) putida or the Glowing gene of Vibrio fischeri. USE/ADVANTAGE - Described is a simple detection method for genetically engineered microorganisms which carry a modified lac repressor gene and which, for example, may have escaped from a laboratory environmentntn
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申请公布号 |
DE4103289(A1) |
申请公布日期 |
1992.08.06 |
申请号 |
DE19914103289 |
申请日期 |
1991.02.04 |
申请人 |
BATTELLE-INSTITUT EV, 6000 FRANKFURT, DE |
发明人 |
MIESCHENDAHL, MARTIN, DR., 5060 BERGISCH GLADBACH, DE |
分类号 |
C12N15/113;C12N15/65;C12Q1/04;C12Q1/68 |
主分类号 |
C12N15/113 |
代理机构 |
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