发明名称 |
CELL ABLATION USING TRANS-SPLICING RIBOZYMES |
摘要 |
The design of new ribozymes capable of self-catalyzed trans-splicing which are based upon the catalytic core of a Group I intron are described. Using this design, it is possible to construct ribozymes capable of efficiently splicing a new 3' exon sequence into any chosen target RNA sequence in a highly precise manner. A method of cell ablation is also described that provides a toxic product to a host cell in vivo in a targetted, regulated manner utilizing novel trans-splicing ribozymes of the invention. Inactive pro-ribozyme forms are also described. |
申请公布号 |
WO9213089(A1) |
申请公布日期 |
1992.08.06 |
申请号 |
WO1992US00277 |
申请日期 |
1992.01.16 |
申请人 |
THE GENERAL HOSPITAL CORPORATION;THE PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
HASELOFF, JAMES;BRAND, ANDREA;PERRIMON, NORBERT;GOODMAN, HOWARD, M. |
分类号 |
C12N1/21;A61K38/00;C07K7/06;C12N;C12N5/10;C12N7/00;C12N9/10;C12N15/09;C12N15/113;C12N15/52;C12N15/66;C12N15/82;C12N15/85;C12N15/90 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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